Abstract
This study focus on developing new salinity tolerance and high yielding rice lines, using markers assisted backrossing (MABC). Total of 500 SSR markers on 12 rice chromosomes were screened for parental polymorphic markers. Of which, 52 primers in the Saltol region were checked with the two parents varieties to identify polymorphic primers for screening the Saltol region of the breeding populations. For each backcross generation of ASS996/FL478, approx. 500 plants were screened with 63 polymorphic markers distributed on 12 chromosomes. The two BC1F1 plants P284 and P307 which had the highest recipient alleles up to 89.06% and 86.36%, were chosen for the next backcrossing. Three BC2F1 plants with the recipient alleles up to 94.03% and 93.18% were used to develop BC3F1 generation. The best BC3F1 plant was P284-112-209 with all the recipient alleles and Saltol region. The four plants P307-305-21, P284-112-195, P284-112-198, P284-112-213 were the second ranking with only one loci heterozygous (applied 63 markers covered on 12 chromosomes). These five plants were chosen as the breeding lines for result of Saltol-AS996 introgression. The breeding line BC4F1 having 100% genetic background of donor variety is ready for develop new salinity tolerant variety ASS996-Saltol to cope with climate change.
Highlights
Rice is the most important food source for half of the world’s population and the main staple food for most of the country’s 86 millions people
The four plants P307-305-21, P284112-195, P284-112-198, P284-112-213 were the second ranking with only one loci heterozygous
The four plants P307-305-21, P284-112-195, P284-112-198, P284-112-213 were the second ranking with only one loci heterozygous
Summary
Rice is the most important food source for half of the world’s population and the main staple food for most of the country’s 86 millions people. Research at IRRI resulted in the development of high yielding rice varieties tolerant of abiotic stresses such as submergence and salt stress, and these varieties can help the unfavorable coastal areas less vulnerable to climate change impacts [1]. These improved varieties were developed using both conventional and modern breeding tools. Introgression the Salinity Tolerance QTLs Saltol into AS996, the Elite Rice Variety of Vietnam. Saltol is a major QTL associated with the Na-K ratio and seedling-stage salinity tolerance, was identified on chromosome 1. An analysis of single feature polymorphism in the Saltol region suggested that FL478 contained a DNA fragment smaller than 1 Mb from Pokkali at 10.6 - 11.5 Mb on chromosome 1 [5]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
