Introduksi Gen DefH9-iaaM dan DefH9-RI-iaaM ke dalam Genom Tanaman Tomat Menggunakan Vektor Agrobacterium tumefaciens

Abstract

<p>Introduction of DefH9-iaaM and DefH9-RI-iaaM Gene<br />Into Tomato Genome Using Agrobacterium tumefaciens.<br />Ragapadmi Purnamaningsih. Plant genetic improvement<br />can be conducted through genetic engineering.<br />Parthenocarpic fruit production could increase fruit<br />production and its qulities. IAA genes were introduced into<br />three tomato cultivars Ratna, Opal and LV 6117 using two<br />constract genes DefH9-iaaM and DefH9-RI-iaaM. The iaaM<br />gene is able to increase auxin biosynthesis in transgenic<br />plant cells and organs because indol-eacetamide,<br />synthesized by the product of the iaaM gene, is converted<br />either chemically or enzimatically to indole-3-acetic acid<br />(IAA), while the promotor DefH9 enable IAA gene expressed<br />specifically in the ovules. The objectives of this experiment<br />was to identify gene introduction into plant genom of three<br />tomato cultivars. The factors tested were two constract of<br />IAA genes (DefH9-iaaM or DefH9-RI-iaaM), tomato cultivars<br />(Ratna, Opal, and LV 6117) and time of explant inoculation<br />(5, 15, 30 minute). The result showed that the best time<br />inoculation was 5 minute. Otherwise three tomato cultivars<br />response better to DefH9-RI-iaaM than DefH9-iaaM. The total<br />efficiency of regeneration and total efficiency of<br />transformation of both genes were 25.38% and 20.32%. PCR<br />analysis showed that 10 plant have positive PCR, were 1<br />plant carried (Opal) DefH9-iaaM gene and 9 plant (Ratna,<br />Opal, LV 6117) carried DefH9-RI-iaaM gene.</p>