Abstract
In this study, we employed an electrochemical (potentiometric) method to enumerate magnetotactic bacteria (MTB) during its coupling with iodometric titration to obtain a selective, precise and rapid counting system. Oxygen was considered as an important factor for the orientation and movement of MTB towards the magnet-modified indicator electrode. In the direct potentiometry, a linear correlation was detected between potentiometric response and dissolved oxygen (DO) concentrations. By the increase of the DO concentration, potential difference would increase in the range of 4.0 to 20.0 parts per million (ppm) at different pressure conditions. The reliability of the O2 bio-sensing feature provides a selective MTB-based cell enumeration methodology based on indirect potentiometric titration. Furthermore, a five-minute H2-purging resulted in an increase of potentiometric response sensitivity arising from the decrease in DO concentration of the electrolyte solution. Results were also investigated by zeta potential difference, which show the effect of charge density of MTB in presence of DO. Zeta potential was increased proportionally by addition of the MTB population. Regarding the reliability of the suggested method, data obtained by the designed system showed no statistical difference from those obtained by the most common procedure in microbiology for enumeration of bacteria, known as colony forming unit (CFU) method.
Highlights
In this study, we employed an electrochemical method to enumerate magnetotactic bacteria (MTB) during its coupling with iodometric titration to obtain a selective, precise and rapid counting system
The aim of our study is to present a highly selective, precise, and cost-effective method for enumeration of the MTB without requiring any time-consuming steps of bacterial purification
A specified procedure for enumoration of magnetotactic bacteria was introduced by combining potentiometry and iodometric titration
Summary
We employed an electrochemical (potentiometric) method to enumerate magnetotactic bacteria (MTB) during its coupling with iodometric titration to obtain a selective, precise and rapid counting system. This assay is often utilized to measure the proliferation and viability of mammalian cells, MTT and other relative materials can be employed for measuring bacterial viability[7] Another procedure to quantify bacterial growth is based on the measurement of the light scattering originating from the turbidity brought about by bacteria measured at 600 nm, referred to as optical density (OD600)[13]. This method is often used as a rapid and cost-effective technique to detect bacterial growth and throughout the culture in broth media. A wide variety of MTB’s applications have been reported so far, there are no reports available for using this type of bacteria in manufacturing a bacterial enumeration system
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