Intrinsic peroxidase-like activity of Cu2ZnSn(SxSe1-x)4 nanocrystals, and their application to the colorimetric detection of H2O2.

Abstract

Nanocrystals (NCs) of type Cu2ZnSn(SxSe1-x)4 (CZTSSe) were prepared via a solvothermal approach. They are shown to be highly efficient peroxidase (POx) mimics for colorimetric detection of H2O2. By varying the molar ratio of S and Se during preparation, the NCs showed different crystal structures, morphologies, surface properties, and POx-like activities. Among them, the type CZTSSe-0.25 NCs exhibit the strongest POx-like activities towards the catalytic oxidation of 3,3',5,5'-tetramethylbenzidine in the presence of H2O2 to generate a blue product. The enhanced activity is attributed to its more negative potential and larger specific surface of the NCs. Based on these findings, a rapid and ultrasensitive method was developed for the visual and colorimetric determination of H2O2. The method is selective, and the NCs are reusable and long-term stable. The detection limit of H2O2 is 50nM. Kinetic and active species trapping experiments were performed to elucidate the POx-like mechanism of the NCs. Graphical abstract Schematic presentation of the process of Cu2ZnSn(SxSe1-x)4 nanocrystals catalyzing the oxidation of peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2 to induce a typical blue color reaction, which can be applied in colorimetric detection of H2O2.