Abstract
Simultaneous monitoring of many functioning β-cells is essential for understanding β-cell dysfunction as an early event in the progression to diabetes. Intrinsic optical signal (IOS) imaging has been shown to allow high resolution detection of stimulus-evoked physiological responses in the retina and other neural tissues. In this paper, we demonstrate the feasibility of using IOS imaging for functional examination of insulin secreting INS-1 cells, a popular model for investigating diabetes associated β-cell dysfunction. Our experiments indicate that IOS imaging permits simultaneous monitoring of glucose-stimulated physiological responses in multiple cells with high spatial (sub-cellular) and temporal (sub-second) resolution. Rapid IOS image sequences revealed transient optical responses that had time courses tightly correlated with the glucose stimulation.
Highlights
Islets of Langerhans are micro-organs in the pancreas that contain several types of endocrine cells, including the β-cells
We demonstrate the feasibility of using Intrinsic optical signal (IOS) imaging for functional examination of insulin secreting INS-1 cells, a popular model for investigating diabetes associated β-cell dysfunction
Our experiments indicate that IOS imaging permits simultaneous monitoring of glucose-stimulated physiological responses in multiple cells with high spatial and temporal resolution
Summary
Islets of Langerhans are micro-organs in the pancreas that contain several types of endocrine cells, including the β-cells. Pancreatic islet β-cells are the only cell type that produces and releases insulin, the major hormone for accurate control of the glucose level in the blood, i.e. glucose homeostasis. Quantitative evaluation of β-cell function is essential for the advanced study and reliable diagnosis of diabetes, and for quality control of isolated islets in clinical transplantation. Optical imaging approaches, including optical coherence tomography (OCT) [4, 5] and confocal microscopy [6], can provide high resolution morphological imaging of pancreatic islets. Morphological and functional abnormalities of islet cells are not always directly correlated
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