Intrinsic noise in locust photoreceptors.

Abstract

1. In locust photoreceptors, the amplitude of the response to light pulses lasting less than 20 ms depends solely upon the number of absorbed photons, which can be estimated at low intensities by counting quantum bumps. Consequently, each receptor can be operated as a calibrated photon counter. 2. Three types of noise in receptor responses have been identified--extrinsic or photon noise and two types of intrinsic noise, dark noise (spontaneous activity) and transducer noise (noise in the transduction mechanism). The methods by which the noise sources are measured and identified involves measuring the responses to a train of flashes of constant intensity and converting these voltage values into a series of equivalent quantum catches. Because photon absorptions follow the Poisson distribution, the variance among equivalent catches due to photon noise equals the mean catch, and any excess variance represents intrinsic noise. 3. Dark noise is negligible: spontaneous signals (quantum bumps produced in darkness) occur less than ten times per hour at 25 degrees C, and the combined effects of membrane and electrode noise are unimportant at all but the highest intensities. 4. At low intensities transducer noise is responsible for more than 50% of all receptor noise (variance), and this rises to 90% when bright stimuli are presented to the dark-adapted eye. 5. Two simple models of transduction indicate that variations in the amplitudes and latencies of responses to single photons are a major source of transducer noise. 6. Transducer noise would be difficult to detect from an analysis of response noise alone, without knowledge of absolute photon catch, because in some important respects it mimics photon noise, e.g. it lowers the quantum efficiency without violating the square root relationship relating increment thresholds to mean intensity.