Abstract
P2 purinoceptor-dependent control of resistance to ischemia reperfusion injuries was studied in Langendorff perfused C57/Bl6 mouse hearts subjected to 20 min ischemia and 45 min reperfusion. Effects of P2 agonism and antagonism were assessed. Control hearts recovered 68±4 mm Hg ventricular pressure (63±3% pre-ischaemia), exhibited sustained diastolic contracture (23±2 mm Hg), and released 26±4 U/g lactate dehydrogenase (LDH) during reperfusion, evidencing oncosis. Treatment with 250 nM of the P2 agonist uridine 5′-triphosphate (UTP) for 10 min prior to and 10 min after ischemia reduced diastolic contracture by ∼50% (9±2 mm Hg), improved pressure development (85±5 mm Hg; 77±2% of baseline), and reduced LDH loss by 60% (11±2 U/g).
Published Version
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