Abstract
Aims/Purpose: The main objective is to describe the inflammatory response produced in the mouse vitreous composition and structure after intravitreal injection (IVI). The mechanism by which a sterile IVI produce inflammation has been previously described; however, no study has analyzed the specific changes occurring at the vitreous fibrils.Methods: Healthy adult male and female C57/BL6J mice were randomized to receive “in vivo” either physiological serum injection (7μl; one dose; n = 6) or no injection (n = 5). After 1 hour and 24 hours, eyes were enucleated, embedded in paraffin, sectioned (5 μm), and analyzed by scanning confocal microscopy after immunohistochemistry.Results: The sterile injection of serum in the mouse vitreous chamber produced noninfectious inflammation, characterized by the invasion of Mac‐2 (CD11b) expressing cells, a marker of macrophages. Furthermore, using anti‐fibrillin 1 antibody, the injected eyes showed an increased vitreous fibril density. Interestingly, macrophages expressed intracellularly fibrillin 1 suggesting that vitreous fibrils could be produced from these hyalocyte‐like cells under inflammation.Conclusions: Understand the composition and spatial arrangement of the vitreous fibrils can be very useful for evaluating pathophysiological conditions during posterior eye diseases. Additionally, determining the role these fibrils play during the post‐IVI inflammatory process provides new insights into the impact of IVI on this part of the eye. These results also establish the mouse as a model suitable for studying the iatrogenic mechanisms associated with therapeutic intravitreal injections.
Published Version
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