Abstract
The conservation of diseases resistant sources of Dalbergia sissoo germplasm has attained the key status among the Agricultural prospects and solution against their possible threats. For this purpose, the best strategy is DNA barcoding based on nuclear, mitochondrial and plastid genomic regions. This approach is frequently used for species discrimination but its potential to discriminate the individuals at intraspecific level could also be worth exploring considerably. Hence, in the present study the potential of rbcL DNA barcode was evaluated for demonstrating intraspecific variation among the shisham genotypes collected across Pakistan. DNA barcoding analysis was performed on 20 selected shisham genotypes. Nucleotide sequences were analyzed to confirm genetic diversity among studied genotypes (θw = 1.000, π = 0.00172). The allelic profile of data yielded 11 unique sequence types (STs) numbers. The phylogenetic tree based on SYM+G model revealed the discriminatory power of rbcL DNA barcode to resolve and differentiate the individuals at intraspecific level. Keywords: Allelic profile, Sequence types, Akaike Information Criterion, Typing efficiency, DNA substitution.
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