Abstract
The environment-dependent multiexponential behavior of N-acetyltyrosinamide (NAYA) and other tyrosine derivatives is revisited, aiming for a better understanding of tyrosine as an intrinsic fluorescent probe for protein microenvironment changes during conformational changes. The effects of solvent polarity, viscosity, and temperature on the fluorescence decay of NAYA were evaluated using dioxane−water mixtures and pure solvents. Double-exponential decays were observed in dioxane−water mixtures above 70% (v/v) water concentration including pure water, for temperatures below 50 °C. However, at higher temperatures, or in dioxane−water mixtures with lower water concentrations, NAYA shows single-exponential decays. Single-exponential decays were also generally observed in pure solvents (dioxane, acetonitrile, methanol, ethanol, DMSO). The exception was the strong hydrogen-bond donor trifluorethanol, in which NAYA decays as a double exponential. The results are consistent with a solvent-modulated excited-state ...
Published Version
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