Abstract
Immunocytochemical localization of glutathione-peroxidase (GSH-PO) in the rat adrenocortical cells was observed not only in cytosol (CYTOSOL GSH-PO) but in mitochondria (MITOCHONDRIAL GSH-PO) by the direct peroxidase labeled antibody method using anti-GSH-PO IgG Fab' fragment. In the untreated rat adrenocortical cells, CYTOSOL GSH-PO was observed mainly in the outer part of the zona fasciculata and zona reticularis. MITOCHONDRIAL GSH-PO was observed mainly in the inner part of zona fasciculata. Biochemical GSH-PO activity in the cytosol and mitochondrial fractions obtained from whole adrenal gland was comparable to the immunocytochemical results. In the hypophysectomized rat adrenocortical cells, markedly atrophic inner part of zona fasciculata showed only CYTOSOL GSH-PO, and biochemical activity of the cytosol GSH-PO was markedly increased. It has been reported by us that this atrophic zone contains accumulated cholesterol esters. By ACTH administration to the hypophysectomized rats, markedly, hypertrophic outer part of zona fasciculata showed increased immunocytochemical localization of MITOCHONDRIAL GSH-PO. And biochemically, mitochondrial GSH-PO activity in this group was higher than that of the hypophysectomized group.These results suggest that the CYTOSOL GSH-PO has its role in the reduction of cholesterol ester hydroperoxides and that the MITOCHONDRIAL GSH-PO in the reduction of sterol hydroperoxides produced during steroidogenesis.
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