Abstract
Adrenal polysomes from control and adrenocorticotropin (ACTH)-treated rats were prepared. Sucrose gradient sedimentation analysis of the free polysome preparation showed an increased yield of monomeric and dimeric ribosomes from ACTH-treated animals. This effect was present at 7 minutes after ACTH injection and disappeared between 60 and 120 minutes of treatment. Similar change was observed in hypophysectomized rats following simultaneous injection of ACTH plus aminoglutethimide (an inhibitor of steroidogenesis) but not after dexamethasone. These increased particles are free of mRNA and peptidyl-tRNA for they dissociate completely by centrifugation through high ionic strength buffer. These results suggest that, in rat adrenal cells, ACTH could act at the level of the protein synthesizing system, most likely at the translational level.
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