Intrafollicular pressure decreases in hamster preovulatory follicles during smooth muscle cell contraction in vitro.

Abstract

Smooth muscle cells in the base of hamster follicles contract minutes prior to ovulation; during this contraction the profile of the follicle changes from low to tall. Our purpose was to measure intrafollicular pressure, independent of an intact circulatory system to the ovary, at the time when follicular smooth muscles are known to be contracting. Hamster ovaries were removed 13 hours after hCG injection and placed in a culture medium which supports in vitro ovulation. Intrafollicular pressure was measured continuously with a WPI Model 900 Micropressure system, while the follicle was being observed and photographed using a dissecting microscope and Zeiss 35 mm camera. Initial intrafollicular pressure was 2.1 +/- 1.1 mmHg (n = 47) in the absence of a blood supply. This pressure decreased slowly (0.03 mmHg/min.) until the transition from a low to tall profile occurred at which time the pressure decreased at a rate of 0.09 mmHg/min. At ovulation, the pressure drops precipitously to 0.0 mmHg. The condition of the follicle, in particular the apex, was examined using scanning electron microscopy (SEM). At 13 hours after hCG, rupture sites had started forming in the apices of 94.3% of the unovulated follicles. In some instances, surface epithelial cells were stretched apart and gaps were present between cells. More often portions of the surface epithelium were missing and the underlying cell layers were exposed. Fluid and erythrocytes were observed passing through the developing rupture sites. Taken together these observations demonstrate that intrafollicular pressure in hamster preovulatory follicles decreases gradually at the time follicular smooth muscle cells contract. We suggest that contraction of follicular SMC is a slow, gradual process which causes formation and/or enlargement of holes in the rupture site. An increase in the size or number of these apertures would lead to increased leakiness and could account for the observed acceleration in the rate of pressure decrease during profile transition. Our observations are the first to correlate changes in intrafollicular pressure with the contraction of smooth muscle cells and condition of the apex.