Abstract
HighlightsVapor nanobubble (VNB) photoporation represents a promising physical technique for mRNA transfection of adherent and suspension cells.A multitude of parameters related to the VNB photoporation procedure were optimized to enable efficient mRNA transfection.VNB photoporation was found to yield five times more living, transfected Jurkat T cells as compared to electroporation, i.e., currently the standard nonviral transfection technique for T cells.
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