Intra-Epidural Space Injection of OX26-PEGylated Selenium Nanoparticles Enhances Motor Function and Decrease the Risk of Neural Damage in Animal Model of Subarachnoid Hemorrhage

Abstract

Objective: To evaluate the effectiveness of PEGylated (PEG) OX26 loaded with Selenium (Se) on locomotor activity and brain function and exploring underlying mechanism in animal model of subarachnoid hemorrhage (SAH). Methods: Thoracis spinal cord injury in Wistar rats was used to induce SAH and intra-epidural injection of OX26-PEG-Se nanoparticles (NPs) was then applied. Locomotor function test was used to evaluate the behavioral outcome in addition, ELISA kit was used to evaluate the serum level of NSE and S100B. Immunofluorescent imaging was used to detect the expression of eNOS and NT-1. In addition, NeuN staing was used to assess the neural damage. Results: The locomotor function of animals with SAH was significantly increase afgter treating with OX26-PEG-Se NPs. In addition, the expression levels of NSE and S100B were significantly decrease after treating animals with OX26-PEG-Se NPs in comparison to sham operated animals. We observed that OX26-PEG-Se NPs decrease the neural damage and the level of NT-1, while increase the eNOS in brain. Conclusion: Intra-epidural injection of OX26-PEG-Se NPs improved the locomotor activity and also inhibit the risk of neural damage through ET-1/eNOS pathway after subarachnoid hemorrhage.