Intestinal versus External Growth Conditions Change the Surficial Properties in a Collection of EnvironmentalEscherichia coliIsolates

Abstract

Predicting the fate of microorganisms in the environment is increasingly warranted, especially for pathogenic strains. A major habitat of Escherichia coli, which encompasses commensal as well as pathogenic strains, is the gastrointestinal tract with conditions very different from the environment it encounters after shedding from the host or during cultivation in the laboratory. We developed two relevant growth conditions representative of intestinal (host-associated) and external (postshedding) environments to investigate the surficial properties and behaviors of a diverse subset of E. coli feedlot isolates. Surficial properties may determine an isolate's physical fate. A pronounced increase in cell hydrophobicity and concomitant biofilm mass formation was observed for isolates grown under external conditions. Isolates that exhibited the highest surface hydrophobicity also formed visible suspended aggregates under external conditions. Other than hydrophobicity, flagella-mediated motility was determinant in affecting E. coli biofilm formation under external conditions, with all four nonmotile E. coli isolates characterized as thin-biofilm formers. The majority (88%) of Ag43+ (outer membrane protein, antigen 43) isolates formed thick biofilms, whereas the majority (75%) of Ag43- isolates formed thin biofilms. The tested E. coli O157:H7 strain behaved differently from the environmental E. coli isolates: it displayed a low electrostatic charge, a small decrease in hydrophobicity upon shifts to external conditions, and very little biofilm formation. On the other hand, the commonly used laboratory strain E. coli K-12 displayed low hydrophobicity both intestinally and externally, but it formed significant biofilm mass under external conditions. Clearly, various E. coli strains manifest significant variability in surficial behavior. This variability is further modulated by growth conditions. The interacting strain-inherent and cultivation-dependent effects on surficial behavior may have broad consequences for the fate and ecology of pathogenic and commensal E. coli strains.