L-Tryptophan prevents Escherichia coli biofilm formation and triggers biofilm degradation

Abstract

The effect of deletion of trp operon and tna operon on the Escherichia coli biofilm formation was investigated in order to elucidate the role of l-tryptophan metabolism in biofilm formation. trp operon deletion mutants ΔtrpC, ΔtrpD and ΔtrpE deficient in l-tryptophan biosynthesis showed higher biofilm formation. In addition, ΔtnaC with increased l-tryptophan degradation activity showed higher biofilm formation. On the contrary, ΔtnaA deletion mutant which lost l-tryptophan degradation activity showed low biofilm formation. From these results, it was suggested that decrease of intracellular l-tryptophan level induced biofilm formation and increase of l-tryptophan repressed biofilm formation. So the effect of the addition of l-tryptophan to the medium on the E. coli biofilm formation was investigated. l-Tryptophan addition at starting culture decreased biofilm formation and furthermore l-tryptophan addition after 16h culture induced the degradation of preformed biofilm. From the above results, it was suggested that maintenance of high intracellular l-tryptophan concentration prevents E. coli biofilm formation and elevation of intracellular l-tryptophan concentration triggers degradation of matured biofilm.