Intestinal toxicity and microbial community disorder induced by bisphenol F and bisphenol S in zebrafish

Abstract

The intestine is the important bioaccumulation and target organ of Bisphenol F (BPF) and Bisphenol S (BPS). Morphological and functional abnormalities induced by BPS and BPF exposure in zebrafish intestine have been reported. However, the underlying mechanisms are not well understood, and the combined toxicities of BPS and BPF in the intestine have not been studied. Here, the zebrafish were treated by single and combined exposure of BPF and BPS at 1, 10, 100, 1000 μg/L. Oxidative damage, inflammation, and transcriptome profiles in the zebrafish intestine were determined. Changes in microbial community structure in zebrafish intestine were analyzed. Results showed that BPF, BPS, and BPF + BPS exposures significantly increased MDA, 8-OHdG, 1L-1β, and TNF-α levels in the zebrafish intestine, indicating oxidative damage and inflammatory effects. Co-exposure of BPS and BPF did not cause synergistic effects on the above effects but induced more changes in gene expression profiles. The changes in the PPAR signaling pathway might be associated with oxidative damage and inflammation. The amino acid metabolism and steroid biosynthesis were specifically altered by co-exposure of BPF and BPS. Moreover, BPF and/or BPS exposures altered microbial community structure in the zebrafish intestine, which showed different influence patterns. Increased abundance of potentially pathogenic bacteria (such as Flavobacterium, Pseudomonas, and Stenotrophomonas) might indicate one of the potential health hazards in zebrafish intestine. The above results provide basic information for the health risk assessment of BPS and BPF in aquatic organisms.