Abstract
The intestinal epithelium forms the inner layer of the human intestine and serves a wide range of diverse functions. Its constant exposure to a vast amount of complex microbiota highlights the critical interface that this single-cell layer forms between the host and our environment. Importantly, the well-documented contribution of environmental factors towards the functional development of the human intestinal epithelium directly implies epigenetic mechanisms in orchestrating this complex interplay. The development of intestinal epithelial organoid culture systems that can be generated from human tissue provides researchers with unpresented opportunities to study functional aspects of human intestinal epithelial pathophysiology. In this brief review, we summarise existing evidence for the role of epigenetics in regulating intestinal epithelial cell function and highlight the great potential for human gut organoids as translational research tools to investigate these mechanisms in vitro.
Highlights
The intestinal epithelium serves a large variety of diverse functions including absorption of nutrients and water as well as forming a critical barrier to the environment [1]
The ability to mediate exposure to environmental factors into potentially stable alterations of cellular function is a hallmark of all epigenetic mechanisms [2]
Their involvement in regulating cellular function of the intestinal epithelium during development and in healthy homeostasis follows as a logical conclusion
Summary
The intestinal epithelium serves a large variety of diverse functions including absorption of nutrients and water as well as forming a critical barrier to the environment [1]. The ability to mediate exposure to environmental factors into potentially stable alterations of cellular function is a hallmark of all epigenetic mechanisms [2] Their involvement in regulating cellular function of the intestinal epithelium during development and in healthy homeostasis follows as a logical conclusion. Given the complexity of environmental factors that might contribute to shaping the intestinal epithelial epigenome in vivo, a reductionist approach may be beneficial in order to identify underlying mechanisms In this respect, the development of three-dimensional organoid models, which closely resemble the in vivo situation, has provided unprecedented opportunities for scientists to investigate fundamental aspects of cell biology. Work from our group and others have started to investigate the use of human IEOs as translational research tools to explore the role of epigenetic mechanisms in GI development, healthy homeostasis, and related diseases [30, 34]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
