Interêt de la détection des papillomavirus humains par hybridation en phase liquide en routine: comparaison avec l'examen cytologique classique pour le dépistage des lésions pré-cancéreuses et cancéreuses du col utérin

Abstract

Human papillomavirus (HPV) infection, especially by oncogenic types is the major cause of human cervical precancerous and cancerous lesions. HPV detection in routine has given rise to numerous contradictory studies using various and different techniques and is still debated. The aim of the present work is to test the reliability of HPV detection for the screening of cervical lesions, in a series of 2385 unselected women attending cervical screening and in 165 fresh cone biopsy samples. For that, a simple, easy to perform liquid hybridization assay in microtiters, Hybrid Capture (HC II, Digene®) has been used to detect 18 HPV types (5 non oncogenic and 13 oncogenic). In our experience, the general sensitivity of HC II to detect high grade lesions is close to 100 %, with a specificity of 84.7 %, a positive predictive value of 15.3 % and a negative predictive value of 100 %. Classic cytologic examination gives a sensitivity of 87.5 % and a specificity of 93.8%. Thus the HC II assay could be used in routine in association with cytology to improve significantly the screening of cervical lesions.