Abstract
High performance liquid chromatography was used to determine interspecific differences in relative concentrations of individual coumarin and flavonoid compounds present in the persistent leaves of six Artemisia L. taxa from subgenus Tridentatae (McArthur), tribe Anthemideae, family Asteraceae. An internal standard technique provided an integrated area profile characteristic of each taxon and allowed relative quantification of up to 34 compounds present in the extracts. Sagebrush samples were collected during 1984 and 1986 from various locations and seasons throughout northern Nevada. Subsequent discriminant analysis performed on relative concentrations among 31 phenolic constituents in the extracts for these six taxa revealed 100% classification agreement to morphological identifications. Two-way (species-by-plants) ANOVA indicated that all significantly variable phenolic concentrations arose from interspecific differences. Interspecific variability in phenolic concentrations among these six taxa appears to be greater than interplant, geographic, or seasonal variation. Whether or not interspecific differences in phenolic concentrations among these six taxa represent genetic or environmental influences has not yet been determined.
Published Version
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