Interplay between the cpSRP pathway components, the substrate LHCP and the translocase Alb3: An in vivo and in vitro study

Abstract

The chloroplast signal recognition particle (cpSRP) and its receptor, cpFtsY, posttranslationally target the nuclear-encoded light-harvesting chlorophyll-binding proteins (LHCPs) to the translocase Alb3 in the thylakoid membrane. In this study, we analyzed the interplay between the cpSRP pathway components, the substrate protein LHCP and the translocase Alb3 by using in vivo and in vitro techniques. We propose that cpSRP43 is crucial for the binding of LHCP-loaded cpSRP and cpFtsY to Alb3. In addition, our data suggest that a direct interaction between Alb3 and LHCP contributes to the formation of this complex. Structured summary MINT- 7992851: Alb3 (uniprotkb: Q8LBP4) physically interacts (MI: 0915) with cpSRP43 (uniprotkb: O22265) by two hybrid (MI: 0018) MINT- 7992897: cpSRP43 (uniprotkb: O22265) and Alb3 (uniprotkb: Q8LBP4) physically interact (MI: 0915) by bimolecular fluorescence complementation (MI: 0809) MINT- 7993251: SRP43 (uniprotkb: O22265) binds (MI: 0407) to LHCP (uniprotkb: P27490) by pull down (MI: 0096) MINT- 7993207: cpSRP43 (uniprotkb: O22265) physically interacts (MI: 0915) with ftsY (uniprotkb: O80842), LHCP (uniprotkb: P27490), SRP-54 (uniprotkb: P37106) and Alb3 (uniprotkb: Q8LBP4) by pull down (MI: 0096) MINT- 7993272: Alb3 (uniprotkb: Q8LBP4) and LHCB (uniprotkb: P27490) physically interact (MI: 0915) by bimolecular fluorescence complementation (MI: 0809) MINT- 7992960: cpSRP43 (uniprotkb: O22265) binds (MI: 0407) to Alb3 (uniprotkb: Q8LBP4) by pull down (MI: 0096) MINT- 7993236: Alb3 (uniprotkb: Q8LBP4) binds (MI: 0407) to LHCP (uniprotkb: P27490) by pull down (MI: 0096) MINT- 7993166: cpSRP43 (uniprotkb: O22265) physically interacts (MI: 0915) with LHCP (uniprotkb: P27490) and Alb3 (uniprotkb: Q8LBP4) by pull down (MI: 0096) MINT- 7993118: cpSRP43 (uniprotkb: O22265) physically interacts (MI: 0915) with Alb3 (uniprotkb: Q8LBP4), SRP-54 (uniprotkb: P37106) and LHCP (uniprotkb: P27490) by pull down (MI: 0096) MINT- 7993046: cpSRP43 (uniprotkb: O22265) physically interacts (MI: 0915) with ftsY (uniprotkb: O80842), SRP-54 (uniprotkb: P37106) and Alb3 (uniprotkb: Q8LBP4) by pull down (MI: 0096) MINT- 7993004: cpSRP43 (uniprotkb: O22265) physically interacts (MI: 0915) with SRP54 (uniprotkb: P37106) and Alb3 (uniprotkb: Q8LBP4) by pull down (MI: 0096)