天麻的抗癲癇作用與Interleukin-1β、Tumor Necrosis Factor-α及一氧化氮關係

Abstract

Purpose Our previous studies have shown that Gastrodia elata (GE) reduces kainic acid (KA)-induced epileptic seizures and inhibits activation of microglia in rates. The present study investigated the relationship between the anticonvulsion effects of GE and interleukin-1β (IL-1β), tumor necrosis factor-α (TNE-α), as well as nitric oxide (NO). Methods Rats were administered KA (12mg/kg) to induce epileptic seizure. After oral administration of GE 1.0g/kg, 0.5g/kg and valproic acid (VA) 250mg/kg for 4 days prior to K injection of the number of KA-induced we dog shakes was calculated, and the changes in IL-1β, TNF-α and NO levels in the peripheral blood, and IL-1β and TNF-α levels in the frontal cortex and hippocampus regions of the rat brain were measured. Results Both pretreatment of GE 1.0g/kg and VA 250mg/kg reduced the number of wet dog shakes. GE also reduced the levels of IL-1β and NO in the peripheral blood, and the levels of IL-1β and TNF-α in the frontal cortex, and TNF-α in the hippocampus. Conclusions GE inhibits the generation of cytokine IL-1β, TNF-α, and NO in rats with KA-induced seizures.