Clinical application of changes of serum cytokines and hypersensitive C-reactive protein in patients with coronary heart disease

Abstract

Objective To study clinical application of the changes of serum cytokines and hypersensitive C-reactive protein (hs-CRP) in patients with coronary heart disease. Methods The levels of interleukin-1β(IL-1β), interleukin-6(IL-6), interleukin-8(IL-8), hs-CRP(with high-sensitive enzyme immunoassay)and tumor necrosis factor α(TNF-α)(with radioimmunoassay) in blood serum were determined in 124 patients with coronary heart disease( including 41 patients with stable angina pectoris(SA), 38 patients with unstable angina (USA) and 45 patients with acute myocardial infarction (AMI)) and 54 normal controls, and then comparatively analysed all the data. Results The levels of IL-1β, IL-6, IL-8 and TNF-α in 124 patients with coronary heart disease were significantly higher than those in 54 normal controls (tIL-1β=3.145, tIL-6=3.627, tIL-8=3.054, tTNF-α=3.301, P＜0.01). The levels of IL-1β, IL-6, IL-8 and TNF-α in 41 patients with SA were no significantly than those in 54 normal controls (tIL-1β= 1.431, tIL-6= 1.587, tIL-8= 1.745, tTNF-α= 1.461, P＞0.05). The levels of IL-1β, IL-6, IL-8 and TNF-α in 38 patients with USA and in 45 patients with AMI were significantly higher than those of in 54 normal controls(USA: tIL-1β=2.871, tIL-6=2.748, tIL-8=2.687, tTNF-α=3.145, P＜0.01; AMI: tIL-1β=2.776,tIL-6=2.874, tIL-8=2.689, tTNFα=3.212, P＜0.01). The level of hs-CRP in SA, USA and AMI groups were higher than thoes in normal controls (tSA=2.617, P＜0.05; tUSA=3.018, P＜0.01; tAMI=3.189, P＜0.01). The levels of IL-1β, IL-6,IL-8, TNF-α and hs-CRP had increasing tendency follow up the severity degree of patients with SA or USA or AMI and the levels of IL-1β, IL-6, IL-8, TNF-α and hs-CRP in patients with AMI were highest in 124 patients with coronary heart disease. Conclusion In the diagnosis of the patients with SA, USA and AMI, the determination of the levels of IL-1β, IL-6, IL-8, TNF-α and hs-CRP in blood serum were important index, and they were references for cardiovascular happened. Key words: Coronary disease; Cytokines; Tumor necrosis factor-alpha; C-reactive protein; Radioimmuno assay;