Interior Temperature and Relative Humidity of an Envelope During Mail Transport by the Japan Post in the Summer: Preliminary Study for a Stability Test of Dried Blood Spot Samples Sent as Regular Mail.

Abstract

The dried blood spot (DBS) is well studied and has been considered a useful technique for collecting biological specimens for therapeutic drug monitoring. Since DBS cards are transported as regular mail, these samples can be exposed to various climatic conditions while in transit. However, there have been limited amounts of data regarding interior temperature and relative humidity (RH) of the envelope during mail transport of DBS samples. This study investigated the interior temperature and RH during the transport of regular mail by Japan Post during the summer and assessed the effect of the gas permeability of zip lock bags on the interior temperature and RH when used as containers for the DBS card. Either an aluminum zip lock bag, gas-impermeable, or a plastic zip lock bag, gas-permeable, containing a desiccant pouch (10 g) and a data logger was packed in an envelope and then transported by mail between Sapporo and Tsukuba/Kagoshima. The ambient temperature and RH in the above cities during the mail transport were obtained from the website of the Japan Meteorological Agency. The envelope was exposed to ≥30°C for up to 22 hours during the mail transport. Differences in the temperature between the interior and exterior of the envelope were almost completely within a range of ±4°C regardless of the gas permeability of the zip lock bags. Although the ambient RH was sometimes over 90% during mail transport, the mean interior RHs of the envelope containing the aluminum or plastic zip lock bag throughout the transport process were 13% and 17%, respectively. Both zip lock bags provided comparable results in relation to the interior temperature and RH of the envelope. Our results suggest that a stability test at 40°C and 90% RH for at least 24 hours is recommended as a validation process if DBS samples are transported as regular mail in Japan. However, conditions may need to be modified depending on the regions.