Abstract
Three interferon-sensitive ( is) mutants of mengovirus have been isolated and the properties of one ( is-1) studied in detail. This mutant, is-1, grows normally in control L cells, but in cells pretreated with interferon, its yield is at least 10-fold lower than wild type ( is +). Furthermore, is-1 attains 95% of its final yield 12 hr postinfection whether in the presence or absence of interferon, whereas in protected cells, is + does not reach this level until 20 hr postinfection. In mixed infection in the presence of interferon, is + can rescue is-1, implying that the mutation affects a diffusible product. Most cells protected with 10 units of interferon and infected with is-1 survive the infection and are able to form clones, as measured by colony-forming ability. If protected cells are treated with actinomycin D immediately following infection, the yields from cells infected with is-1 are enhanced and the yields from cells infected with is + are lowered, the net result being complete reversal of the is-1 phenotype. These results are consistent with the hypothesis that the is-1 function protects the virus genome from an induced cellular nuclease.
Published Version
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