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Abstract
The effects of priming mouse cells with interferon on the production of interferon and its mRNA were investigated. Interferon-treated (primed) mouse L929 cells produce 3 to 10 times more interferon than do nonprimed cells following induction with Newcastle disease virus. Interferon appears 2 to 4 h sooner in the primed cultures than in nonprimed cultures and interferon production by primed cells becomes resistant to inhibition by actinomycin D about 4 h sooner than interferon production in nonprimed cells. Interferon mRNA is detected in primed-induced cells about 2 h earlier than in nonprimed-induced cells. It reaches peak levels about 2 to 4 earlier in primed cells, but it also disappears sooner in primed cells. The total amounts of interferon mRNA isolated from primed-induced cells and nonprimed-induced cells were indistinguishable, by the methods utilized. Therefore, although primed cells can produce significantly more interferon and make interferon mRNA sooner than nonprimed cells, the total amount of interferon mRNA produced is apparently not increased, nor is its half-life prolonged in primed cells. Thus, enhanced interferon production in primed cells may result from enhanced efficiency of translation of interferon mRNA in the primed cells.
Highlights
The effects of priming mouse cells with interferon on the production of interferon and its mRNA were investigated
In the present studies and others [3, 13,14,15,16], interferon-primed cells have been observed to produce interferon sooner than nonprimed cells (Fig. I), and the interferon production in primed cells has been observed to become resistant to inhibition by actinomycin D more quickly
We have asked whether the increased amount of interferon produced in primed cells results from increased interferon mRNA production, from increased interferon mRNA half-life, or from enhanced translation efficiency of this mRNA
Summary
The effects of priming mouse cells with interferon on the production of interferon and its mRNA were investigated. L cells cannot produce interferon in response to polyribonucleotides unless treated with DEAE-dextran [2, 7] This suggests that interferon priming can alter the initial interaction of cells with inducers. It has been repeatedly demonstrated that cells treated with interferon can produce more interferon than untreated cells, when exposed to viral or nonviral interferon inducers [1, 8,9,10,11,12]. ROl-CA-20863 awarded by the National Cancer Institute, by Institutional Core Grant NCI-CA-08748, and by National Institutes of Health Grant ROl-GM-24442
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