Abstract
This chapter discusses the phenomenon of biochemical interference, the role of sample quality on the analysis, and the effect of biological variation in the reported values. Serum or plasma, prepared with various anticoagulants, are used in clinical chemical analysis. The isoenzymes of 6-phosphogluconate dehydrogenase that are determined by electrophoresis on starch gel, but not on agarose, differ according to the anticoagulant used for collecting blood. Many constituents—such as magnesium, potassium, phosphorus, and enzymes—are present in the formed elements of blood, in concentrations many times higher than in the surrounding plasma. Thus, the lysis of cells can contaminate the plasma or serum to a measurable amount. The red cells can release material that interferes in the analytical procedure by contributing color to the reaction, as occurs in the biuret determination of protein, or direct interference in a chemical reaction, as in uric acid assays, which depend on phosphotungstate reduction.
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