Interference with the Expression of miR-93 Inhibits the Proliferation and Migration and Promotes Apoptosis of Thyroid Tumor Cells Through Targeting Large Tumor Suppressor Molecule 2

Abstract

Thyroid cancer (TC) is a common malignant tumor of the endocrine system. Currently, it is an urgent problem to be solved to explore the relevant molecular targets and molecular mechanisms for the occurrence and development of TC. miR-93 plays a role in regulating tumors in multiple cancers, but its expression in TC and its effect on proliferation,migration and apoptosis of TC cells have not been reported so far. In this paper, RT-qPCR was used to detect the expression of miR-93 and LATS2. Cell transfection techniques was used to interfere with or overexpress miR-93 and interfere with the expression of LATS2, and RT-qPCR was used to detect transfection efficiency. CCK-8 technique and clone formation assay were used to detect cell survival. Transwell and wound healing assay was used to detect cell migration and invasion, and western blot was used to detect the expression of invasion and migration related proteins MMP2 and MMP9. The apoptosis rate was detected by flow cytometry, and the expression of apoptotic proteins bcl-2, Bax, caspase3/9 and pro-caspase-3/9 was detected by western blot. The results showed that miR-93 was significantly increased in TC cell lines, especially in TPC1 cells. Interference with the expression of miR-93 inhibited proliferation, invasion, and migration, and promoted the apoptosis of TPC1 cells. In addition, we also found that interfering with the expression of miR-93 can promote the expression level of LATS2 in TPC1 cells. Interference with the expression of LATS2 can inhibit the effect on the proliferation, invasion, migration and apoptosis of TPC1 cells after miR-93 interference. In conclusion, interference with the expression of miR-93 inhibits the proliferation and migration and promotes apoptosis of TC cells through targeting LATS2.