Abstract
Objective To investigate the expression of c-FLIP in gallbladder cancer and explore the effect of siRNA(small interfering RNA) to interfere expression of c-FLIP on gallbladder cancer cell line. Methods The expression of c-FLIP was detected by immunohistochemical method in 35 cases of gallbladder cancer compared with 10 cases of normal gallbladder tissue and 10 cases of gallbladder adenoma. After being interfered by RNAi of c-FLIP, cell viability was measured by CCK-8 method, morphological changes observed through phase-microscopy and apoptosis detected by flow cytometry. Results The positive expression of e-FLIP in gallbladder cancer tissues was significantly higher than that in normal gallbladder tissues(P<0.05) and gallbladder adenoma tissues(P<0. 05). After being interfered by RNAi of c-FLIP, cell growth of gallbladder cancer cell line SGC-996 was markedly inhibited. Either in phase-microscopy or in flow cytometry, the proportion of apoptosis in samples treated with RNAi of c-FlIP for 24 hours was notablely increased as compared with those untreated samples(P<0.05). Conclusion Targeted therapy of RNAi of c-FLIP might be a promising method to treat gallbladder cancer. Key words: Gallbladder neoplasm; c-FLIP; Apoptosis; Immunohistoehemistry; siRNA
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