Abstract
Bioelectrodes mediated by metal oxide nanoparticles have facilitated the development of new sensors in medical diagnosis. High-purity TiO2 nanoparticles (NPs) were synthesized through thermal plasma and deposited directly on an interdigitated electrode. The surface of the TiO2-deposited electrode was activated with (3-aminopropyl) triethoxysilane (APTES) followed by fixing the single-stranded probe deoxyribonucleic acid (DNA) to fabricate the DNA biosensor. The structural properties of the deposited TiO2 nanoparticles were analyzed using a transmission electron microscope (TEM), X-ray diffraction (XRD), and a dynamic light scattering (DLS) system. The chemical composition and structural properties of the TiO2 nanoparticle layer and the fixed layer were analyzed by X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). E. coli O157:H7, a well-known pernicious pathogenic bacterial species, was detected as a target DNA of the prepared DNA biosensor, and the characteristics of DNA detection were determined by the current change using a picoammeter. The degree of binding between the probe DNA and the target DNA was converted into an electrical signal using the picoammeter method to quantitatively analyze the concentration of the target DNA. With the specificity experiment, it was confirmed that the biosensor was able to discriminate between nucleotides with mismatched, non-complementary, or complementary sequences.
Highlights
IntroductionDue to the food poisoning epidemic that is spreading worldwide, more and more attention is being paid to public health issues
A high-purity TiO22-NPs-based microchip biosensor array plasma-deposited on Pt for the detection of E. coli O157:H7 deoxyribonucleic acid (DNA) hybridization was successfully fabricated
The biosensor was fabricated by synthesizing2 TiO2 nanoparticles in thermal plasma, dethem directly onto a Pt interdigitated electrodes (IDEs), and silanizing them to combine single-stranded DNA
Summary
Due to the food poisoning epidemic that is spreading worldwide, more and more attention is being paid to public health issues. The most well-known causes of food poisoning are pathogenic bacteria including Escherichia coli (E. coli) O157:H7, Clostridium perfringens, Salmonella, Campylobacter, Listeria monocytogenes, etc. E. coli O157:H7 was first discovered in 1982 and classified as an enterohemorrhagic E. coli (EHEC), which is a serotype bacterial species and can produce Shiga-like toxins [5,6,7]. It is the causative agent of food-borne diseases caused by contaminated raw food, including unsterilized milk or raw ground beef [8,9]. As the incidence of bacteria-related diseases and the number of deaths continue to increase worldwide, there is an urgent need to develop a biosensor capable of quickly and inexpensively detecting pathogenic bacteria such as E. coli O157:H7 with high sensitivity and specificity
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