Abstract
We offer a highly sensitive and reproducible dielectric-spectroscopy assay of deoxyribonucleic acid (DNA) sequence on a platform of quantum graphene-like structures arranged on nanoporous alumina to correctly identifying an infectious agent in a native double-stranded (ds) DNA. The hybridization of complementary target DNA with probe DNA in the sensor sensitive layer leads to penetration of the formed single-stranded (ss) target DNA into the underlayer nanoporous anodic alumina through the nanocavities of LB-film from organometallic complexes. This results in linking of MWCNT ends, shielding of Helmholtz double layer and following decrease of electrical capacitance of the sensor. The novel electrochemical impedimetric DNA sensor with self-organized multi-walled carbon nanotube (MWCNT) bundles decorated by organometallic complexes as transducer has been utilized to detect the viral DNA in the biological samples of patients with virus infection at DNA concentration as low as 1.0–1.3 ng/μL.
Highlights
Advances in molecular biology in recent decades are connected with utilizing third- 28 generation deoxyribonucleic acid (DNA)-nanosequencing label-free methods [1]
DNA/multi-walled carbon nanotube (MWCNT) and oligonucleotide/MWCNT were obtained by means of ultrasonic treatment of alcoholic solution of ds-DNA or oligonucleotide with MWCNT [6]
The resulting mixtures were homogenized by ultrasonic treatment to form hydrophilic or hydrophobic micelles of stearic acid with complexes ds-DNA/MWCNT or oligonucleotide/MWCNT inside them [12]
Summary
Advances in molecular biology in recent decades are connected with utilizing third- 28 generation DNA-nanosequencing label-free methods [1]. High-sensitive methods to detect viral infection is challenge. R To reveal human parvovirus infection for medical practice we offer a dielec- 38 tric spectroscopy method based on highly selective hybridization interactions of 39. Interacting probe ss-DNA and target genomic linear ss-DNA of samples under investigation form. D a ds-DNA helix of homoduplex on sensor surface for detection of parvovirus infection. UN We utilized two types of ss-DNA probes to recognize parvovirus sequences: direct primer sequence to 3 → 5 –ss-DNA-chain and revers one to 5 → 3 –ss-DNA- chain. The ds-DNA samples have been obtained from blood serum of patients with parvovirus infection (DNApvi, i = 1, 2, 3) and of practically healthy donors (DNAhi, i = 1, 2, 3) as negative control.
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