Abstract

Measurements of primary biological aerosol particles (PBAPs) have been conducted worldwide using ultraviolet light-induced fluorescence (UV-LIF) spectrometers. However, how these instruments detect and respond to known biological and non-biological particles, and how they compare, remains uncertain due to limited laboratory intercomparisons. Using the Defence Science and Technology Laboratory, Aerosol Challenge Simulator (ACS), controlled concentrations of biological and non-biological aerosol particles, singly or as mixtures, were produced for testing and intercomparison of multiple versions of the Wideband Integrated Bioaerosol Spectrometer (WIBS) and Multiparameter Bioaerosol Spectrometer (MBS). Although the results suggest some challenges in discriminating biological particle types across different versions of the same UV-LIF instrument, a difference in fluorescence intensity between the non-biological and biological samples could be identified for most instruments. While lower concentrations of fluorescent particles were detected by the MBS, the MBS demonstrates the potential to discriminate between pollen and other biological particles. This study presents the first published technical summary and use of the ACS for instrument intercomparisons. Within this work a clear overview of the data pre-processing is also presented, and documentation of instrument version/model numbers is suggested to assess potential instrument variations between different versions of the same instrument. Further laboratory studies sampling different particle types are suggested before use in quantifying impact on ambient classification.

Highlights

  • Primary biological aerosol particles (PBAPs) vary in size and abundance, and comprise viruses (0.01–0.3 μm), bacteria and associated agglomerates (0.1–10 μm), fungal spores (1–30 μm), pollen (5–100 μm) and fragments such as plant and animal debris [1,2]

  • Comparisons are conducted for the Wideband Integrated Bioaerosol Spectrometer (WIBS) and Multiparameter Bioaerosol Spectrometer (MBS) instruments, respectively, and their response to bacteria (Section 5.2.1), fungal spore material (Section 5.2.2), pollen and pollen fragments (Section 5.2.3) and non-biological samples (Section 5.2.4)

  • The relationship among particle size, shape and fluorescence is presented in Section 5.3 with the relationship between particle size and shape shown for each instrument and particle type group (Section 5.3.1)

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Summary

Introduction

Primary biological aerosol particles (PBAPs) vary in size and abundance, and comprise viruses (0.01–0.3 μm), bacteria and associated agglomerates (0.1–10 μm), fungal spores (1–30 μm), pollen (5–100 μm) and fragments such as plant and animal debris [1,2]. The dispersal of biological particles in the atmosphere has been found to impact human, animal, and agricultural health [3,4,5] whilst affecting the climate and hydrological cycle, by acting as ice nuclei or cloud condensation nuclei [6,7,8]. Spectrometers, laboratory studies quantifying the response of these instruments to known biological and non-biological particle types are limited. Without such studies, assessing and quantifying the emission and impact of these particles on the environment, and plant and human health, will remain restricted.

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