Abstract
The tear film lipid layer (TFLL) is a multilayer structure of lipids that separate the aqueous tear film covering the eye's cornea from the external environment. Based on lipidomics, it contains different lipid species, in particular, wax esters and cholesterol esters. Structurally, TFLL consists of a relatively thick nonpolar lipid layer spread over a monolayer of polar lipids, the latter residing at the aqueous tear subphase. The aqueous tear film and TFLL constitute a barrier protecting against pollution, microorganisms, and other factors.
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