Abstract
In this study, using pathogenic and non-pathogenic bacteria, it was analyzed whether a polyclonal serum and a monoclonal antibody to A. culbertsoni mannose-binding protein (MBP) could inhibit its interaction. The association of the amoeba with E. coli O157:H7 was very strong at a level of over 100%, but the non-pathogenic E. coli strain was about five times lower at 22%. Pathogenic K. pnueumoniae also showed high association with amoeba by about 92% as compared with pathogenic E. coli O157:H7 and S. agalactiae. The polyclonal serum to MBP inhibited E. coli O157:H7 association to amoeba 2.5 times more than untreated E. coli O157:H7. Monoclonal antibody to MBP also inhibited bacterial association with amoeba but was not stronger than the polyclonal serum. Pathogenic E. coli O157:H7 showed about 88% invasion into amoeba and decreased about 22% as compared with associated E. coli O157:H7. Polyclonal serum to MBP inhibited about 55%, 50%, and 44% in E. coli O157:H7, K. pneumoniae and S. agalactiae, respectively. The invasion of K. pneumoniae and S. agalactiae was not high as polyclonal serum but was about 8% to 10% weaker than polyclonal serum. The pathogenic strains of K. pneumoniae and S. agalactiae showed less decrease in survival as shown at invasion than E. coli O157:H7 without antibody. This study provided the information that the pathogenic bacteria could be more interactive with A. culbertsoni trophozoites as a reservoir host than non-pathogenic E. coli, and the amoeba should interact with bacteria by the MBP lectin.
Highlights
Acanthamoeba is a free-living protozoa isolated in a variety of environments, including air, soil, tap water and swimming pools
This study provided the information that the pathogenic bacteria could be more interactive with A. culbertsoni trophozoites as a reservoir host than non-pathogenic E. coli, and the amoeba should interact with bacteria by the mannose-binding protein (MBP) lectin
In this study, using pathogenic and non-pathogenic bacteria such as E. coli O157:H7, K. pnuemoniae, S. agalactiae and E. coli DH5α, it was analyzed whether a polyclonal serum and a monoclonal antibody to MBP of A. culbertsoni could inhibit its interactions, such as bacterial association, invasion and survival
Summary
Acanthamoeba is a free-living protozoa isolated in a variety of environments, including air, soil, tap water and swimming pools. Because the process of infection between amoebae and macrophages is very similar, amoeba is a powerful model for studying bacteriamacrophage interactions[8,9] Their interactions were inferred from the phagocytic ability of Acanthamoeba and macrophage. Mannose-binding protein (MBP) of A. castellanii and A. culbertsoni, was known to play an important role in the pathogenesis of infection by mediating the attachment of parasites to the host cells[12,13]. It may mediate the interaction of amoeba with other cells such as bacteria. The amount of difference between interactions were analyzed as compared with interaction untreated with antiserum or antibody
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