Abstract
Heterotrimeric Go bound to the membranes of bovine brain, but Goα remained bound to the membranes even after activation with GTPγS. Furthermore, Goα bound to a Triton X-100-insoluble fraction of the membranes in a saturable manner. However, the 37-kDa Goα eliminated by trypsin at the amino-terminus could not bind to the fraction. Using a blot overlay approach of the Triton-insoluble fraction, only a 20-kDa protein was identified that interacts with Goα. These results indicate that Goα binds to a 20-kDa Triton-insoluble protein in the bovine brain membranes.
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