Abstract
Apparatus for coupled high-performance gel permeation chromatography (HPLC) and capillary electrophoresis was constructed and applied to the analysis of complex protein mixtures. To avoid electric leakage to the HPLC system, an electromagnetic pinch valve was inserted in the line from the column outlet to the injection port of the capillary electrophoresis apparatus. All the procedures for chromatography and electrophoresis were automated with the aid of a system controller. The apparatus was successfully used for the automatic two-step separation of human serum proteins and water-soluble proteins in bovine brain. Proteins separated by gel permeation HPLC were further analysed by capillary electrophoresis according to their characteristic electrophoretic mobilities.
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