Interaction of rice salinity screening in germination and seedling phase through selection index based on principal components

Abstract

Salinity stress can reduce rice (Oryza sativa L.) productivity and cause crop failure. This problem needs a solution by development of tolerant varieties, and this development closely relates to the effectiveness of its screening. Screening at the phase of germination and seedling are the most common ones. However, the interactions between screenings based on the growth character selection index have not been widely reported, particularly with the principal component analysis (PCA). Therefore, the aim of this study was to determine the interaction between phases of rice salinity screening through a selection index based on PCA. This study consisted of two phases of salinity screening, namely growth phase of germination and seedlings. Both screenings were designed with a nested randomized complete block design, where replicates were nested in a selection environment. The selection environment consisted of two levels, normal (0 mM NaCl) and saline (120 mM NaCl). The genotypes consisted of eight varieties and was repeated three times. Observations were based on morphological and physiological characters, especially in seedling phase screening. Results showed that morphological character approach of the seedlings had a large distribution of salinity tolerances. The use of stress tolerance index and PCA were considered effective in the formation of the selection index on salinity screening. As for, the morphology index was formulated as 0.32 shoot height + 0.33 root fresh weight + 0.33 shoot fresh weight + 0.26 root length + 0.01 number of tillers, length + 0.34 total biomass fresh weight. Therefore, the use of this analytical concept is recommended in screening the tolerance of rice lines to salinity stress.