Interaction of microRNA-29b/c and myeloid cell leukemia-1 in gastric cancer

Abstract

Objective To study the interation of microRNA(miR)- 29b and miR-29c and myeloid cell leukemia-1(Mcl-1)in gastric cancer. Methods Real-time quantitative polymerase chain reaction(Real-time PCR)and Western blotting were used to detect the expression of Mcl-1 mRNA and protein in gastric cancer cells when miR-29b/c were highly expressed. Vector constuction and Luciferase reporter assay system were applied to verify the relationship between miR-29b/c and Mcl-1. DAPI staining was used to detect the effect of miR-29b/c on apoptosis of gastric cancer cells. Results miR-29b/c could downregulate the expression of Mcl-1 mRNA(0.860±0.006 vs.0.513±0.061, P< 0.05 and 0.860±0.006 vs.0.512±0.104, P< 0.01)and also protein expression.miR-29b/c directly regulated Mcl-1 gene in gastric caner cells(1 234 000±42 720 vs.54 900±13 700,P< 0.01 and 652 700±14 010 vs.283 100±12 750,P< 0.01). Overespression of miR-29b/c in gastric cancer cells signifcantly elevated the apoptosis rate[(1.961±0.476)% vs.(6.319±1.725)%, P< 0.05 and(1.961±0.476)% vs.(4.878±0.614)%, P< 0.01]. Conclusion miR-29b/c can regulated Mcl-1 in gastric cancer cells, which may be one of the pathogeneses of gastric cancer. Key words: Stomach neoplasms; MicroRNA; Myeloid cell leukemia-1; Apoptosis