Study on the role of microRNA-130b in gastric cancer BGC-823 cell proliferation and apoptosis via the phosphatase and tensin homologue deleted on chromosome ten/protein kinase B pathway

Abstract

Objective To investigate the effect of microRNA (miRNA, miR)-130b on the proliferation and apoptosis of gastric cancer cell. Methods MiR-130b expression was examined by quantitative real-time PCR (qPCR)using 38 gastric cancer and adjacent tissues. Cell proliferation and apoptosis and cell-cycle were evaluated by cell counting kit-8 (CCK-8) and flow cytometric assays. Phosphatase and tensin homologue deleted on chromosome ten (PTEN) as a direct target of miR-130b was identified by dual-luciferase reporter assay. PTEN, total protein kinase B (Akt) and p-Akt were assessed by Western blotting. Results MiR-130b highly expressed in gastric cancer compared with adjacent tissue (8.048±0.508, 2.689±0.243, P 0.05), there were significant differences between the two groups (0.469±0.099 vs. 0.920±0.045, P<0.01). MiR-130b inhibitor enhanced expression of PTEN (2.067±0.120, P<0.01)and reduced p-Akt expression (0.501±0.115, P<0.05), miR-130b mimic reduced expression of PTEN (0.526±0.157, P<0.05)and enhanced p-Akt expression (1.860±0.222, P<0.05), while total Akt expression remained unchanged. Conclusion MiR-130b regulates proliferation and apoptosis of gastric cancer BGC-823 cell through PTEN/Akt pathway. Key words: Gastric cancer cell; MicroRNA-130b; Proliferation; Apoptosis; Phosphatase and tensin homologue deleted on chromosome ten/Protein kinase B