Abstract
Abstract Phenolic metabolites from oregano and related species in the family Lamiaceae are important sources of antimicrobials and antioxidants. The content of phenolic metabolites in oregano and related species are highly variable due to genetic heterogeneity. This genetic heterogeneity is due to the breeding character being influenced by natural cross‐pollination. In order to develop gene pools to improve ingredient quality and quantity genetically uniform shoot‐based clonal lines were isolated using plant tissue culture techniques. Clonal lines were generated from multiple shoots induced by 1 mg/1 benzylaminopurine in standard Murashige and Skoog medium with 3 % sucrose. Under these optimum conditions 7–10 shoots per explant were generated for further clonal propagation or regeneration of plants. Shoot‐inducing hormones like thidiazuron and adenine sulfate did not improve multiple shoot‐forming ability. Individual shoots of several individual clonal lines with each originating from a single heterozygou...
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