Abstract
ABSTRACT Oregano, Origanum vulgare, is a source of essential oils and phenolic metabolites, such as rosmarinic acid, which exhibits anti-oxidant and antimicrobial activity. This suggests that the plant material could be useful in food preservation, and in nutraceutical and cosmetic applications. Due to natural cross-pollination, however, oregano has substantial plant-to-plant variation and therefore a considerable genetic inconsistency in the type and level of phytochemicals produced. In this study, clonal lines developed from a population of heterozygous seeds using tissue culture were screened for desirable traits (multiple shoot formation and enhanced biomass, and rosmarinic acid and phenolic acid production) based on tolerance of the clonal lines to Pseudomonas and azetidine-2-carboxylate (A2C). Of the 20 clonal lines isolated, two over-expressed phenolic acids in response to both Pseudomonas and A2C.
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