Interaction of glial and astrocytoma c6 cells in monolayer culture

Abstract

The separation and culture of the glial monolayer cells have been reported. Using the differential attachment method and treatment with cytosine arabinoside (Ara-C) followed by subculturing, a very pure population of glial cells can be achieved. This paper involves the study of the interaction of the glial cells with a tumour cell line C6 astrocytoma (American Type Cell) derived from the mouse brain. In addition the differences in surface morphology of the glial cells and C6 astrocytoma are also included.The C6 cells were cultured in a plastic culture disk using F-10 medium supplemented with 15% fetal bovine serum, 2.5% horse serum, and 1% penicillin and streptomycin (Microbiological Association). The culture medium was changed every 3-4 days. Pure glial cell cultures were prepared as reported, and confluence monolayer glial cells were obtained about one month after subculturing of the primary glial cultures.