Abstract
This study investigated the cytoprotection effect of mulberry juice (MJ) anthothyanin on neuronal glial cell. The results showed that freeze dried mulberry juice powder (MJP) contained 19 mg /g anthocyanin. After purified with solid phase extraction device, anthocyanin content in freeze dried anthocyanin extract from mulberry juice (AEMJ) increased to 243 mg/g. The HPLC analysis revealed that cyanindin-3-glucoside and cyanidin-3-rutinoside are the major anthocyanin in AEMJ. The results of in vitro antioxidant test revealed that the DPPH free radical scavenging ability of MJP achieved to 81% at a concentration of 4 mg/ml. The DPPH free radical scavenging ability of 1 mg/ml AEMJ achieved to 90%, higher than that of catechin at the same concentration. The reducing power of MJP increased with the treated concentration. The absorption value was about 1.58 with the treated concentration of 2 mg/ml. The reducing power of AEMJ, similar to catechin, achieved to 2 at a concentration of 0.25 mg/ml. The rat glial tumor C6 cell was used for investigating the cytoprotection ability of MJP or AEMJ under hypoxia condition induced with Na2S2O4. The cell viability was 60.98 % for hypoxia rat glial tumor C6 cell control group at Na2S2O4 concentration of 5 mM. The cell viability of hypoxia C6 cells was 89.74% when pre-incubated with MJP contains 25 μg /ml anthocyanin. The cell viability of hypoxia C6 cells was 92.21% when pre-incubated with AEMJ contains 50 μg /ml anthocyanin. The intercellular antioxidant glutathione of hypoxia C6 cells increased, however, the superoxide dismutase activity of hypoxia C6 cells decreased, when pre-cultured with AEMJ. The results indicated that both MJP and AEMJ possess cytoprotection ability on hypoxia rat C6 glial cells.
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