Abstract
Aromatic cytokinins (ortho-topolin riboside, 6-benzylaminopurine riboside and 6-(2-hydroxy-3-methoxybenzyla mino)purine riboside) were tested for their possible interaction with human liver microsomal cytochromes P450 by absorption difference spectroscopy. All three compounds were shown to bind to the CYP enzymes producing a high to low spin shift of the heme iron yielding a Soret absorption band shift to approximately 425 nm. As this type of spectral change means that the substance is able to bind directly to the heme iron, the results obtained open the possibility of an interaction of these compounds with metabolism of other drugs or, in general, with other substrates of cytochromes P450.
Highlights
MATERIAL AND METHODSCytokinins are low molecular weight substances (N6substituted adenine derivatives) found in plants
We investigated whether the compounds studied are able to interact with cytochromes P450 (CYP) enzymes present in human liver microsomes
Difference spectra of interactions of cytokinins with microsomal CYP enzymes were followed according to established procedure[6]
Summary
MATERIAL AND METHODSCytokinins are low molecular weight substances (N6substituted adenine derivatives) found in plants. We have performed a pilot study of the interaction of a new class of cytokinin derivatives with cytochromes P450 (CYP). There are about eight CYPs responsible for over 95 % of known cases of drug-dependent cytochrome P450 metabolism: CYP3A4, CYP2D6, CYP2C9, CYP2E1, CYP2C19, CYP1A2, CYP2A6 and CYP2B65.
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