Interaction of abscisic acid and jasmonic acid on the inhibition of seed germination and the induction of freezing tolerance

Abstract

The possible interaction of the two growth regulators, abscisic acid and jasmonic acid, on the inhibition of seed germination and the induction of freezing tolerance in bromegrass (Bromus inermis Leyss) cell cultures was investigated. Both of these processes are known to be affected by exogenous abscisic acid. Alfalfa (Medicago sativa), cornflower (Centurae gynura), cress seed (Lepidium sativum), maize (Zea mays), and wheat (Triticum aestivum) seeds were treated with varying concentrations of abscisic acid and jasmonic acid, either alone or in combination. In all species, seed germination was inhibited by 10 μM abscisic acid at 23 °C. In contrast, at 23 °C, jasmonic acid was partially inhibitory only at 100 μM; however, 10 μM jasmonic acid inhibited germination in all species at 10 °C. Jasmonic acid in combination with abscisic acid resulted in a higher degree of germination inhibition at 23 °C in all species than either growth regulator applied separately. Treatment of a bromegrass suspension cell culture with 75 μM abscisic acid at 25 °C for 7 days increased the freezing tolerance from −10 °C to lower than −35 °C. In contrast, jasmonic acid (0.25–75 μM) had no detectable effect on freezing tolerance. Jasmonic acid in combination with suboptimal concentrations of abscisic acid, however, enhanced the abscisic acid-induced freezing tolerance in these cells. In contrast, a combination of 75 μM abscisic acid and 25 or 75 μM jasmonic acid reduced the freezing tolerance of these cells compared with treatment with abscisic acid alone. Key words: abscisic acid, freezing tolerance, germination, jasmonic acid.