Abstract
1 [Ala3, Ala7] TANDEM is an analogue of des-N-tetramethyl-triostin A (TANDEM) in which both L-Cys residues of the octapeptide ring are replaced by L-Ala; accordingly it lacks the disulphide cross-bridge which limits the conformational flexibility of TANDEM. 2 In [L-Ser1] TANDEM the configuration of one of the serine residues is inverted, altering the disposition of one of the quinoxaline chromophores with respect to the peptide ring. 3 Both compounds interact weakly but detectably with natural DNAs as judged by spectral shifts and increases in the thermal denaturation ('melting') temperature Tm. They also raise the Tm of poly rA . poly rU. 4 Binding isotherms determined by solvent partition analysis with [Ala3, Ala7] TANDEM yield association constants of about 10(3) M-1 for its interaction with natural DNAs. A Scatchard plot for binding to poly(dA-dT) determined by solvent partition and spectrophotometric methods shows marked evidence of cooperativity with an intrinsic association constant 1.9 x 10(4) M-1, 8.7 nucleotides per binding site, and cooperativity parameter 15. 5 Binding of [Ala3, Ala7] TANDEM to short rod-like fragments of poly(dA-dT) increases their contour length by almost the theoretical amount expected for an ideal process of bifunctional intercalation. 6 No effect of either compound on the winding of the DNA helix could be detected in sedimentation experiments with closed circular duplex PM2 DNA. 7 It is concluded that the cross-bridge of TANDEM greatly stabilizes its binding to DNA, most probably via entropic factors, but is not the only structural feature that influences its AT sequence-selectivity. The consequences of epimerising one of the D-Ser residues appear as disastrous as epimerising both. 8 The experimental details for the synthesis of [Ala3, Ala7] TANDEM and [L-Ser1] TANDEM are given in an appendix to this paper.
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