Abstract
In order to understand the role of phospholipids in biological membranes, it is very important to be able to determine whether specific proteins and specific phospholipids can come into contact. This relates to the problem of the specificity and on the frequencies of the interactions between phospholipids and proteins. We will show here that these problems can be approached by the spin-label technique, if one takes advantage of the spin-spin interactions between spin-labeled proteins and spin-labeled phospholipids. Rhodopsin, an intrinsic protein, is chosen to study protein-lipid interactions. However line-broadening due to spin-spin interactions between spin-labeled lipids and spin-labeled rhodopsin could only be seen in bleached rhodopsin (opsin) preparations. Therefore these spin-label experiments give some insight not only into protein-lipid interactions but also into the localization of sulfhydryl sites of rhodopsin and on the modification of the configuration of the protein under the influence of light.
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