Interaction between SIRT-1 Solymorphism and Life-Style on Anthropometric and Biochemical Parameters in Obese Phenotypes

Abstract

<p><strong>Background and Objective:</strong> Sirtuin 1 (SIRT1) is nicotinamide adenine dinucleotide-dependent histone deacetylase. It plays a crucial role in the regulation of fat and glucose metabolism by influencing proteins involved in nutrient sensing and energy regulation. This study examined the effects of the interaction between the SIRT1 rs7069102 variant and lifestyle factors on obesity-associated anthropometric and metabolic phenotypes in the local population of Pakistan.<br /><strong>Methods:</strong> The study population incorporated 612 participants comprising an equal number of individuals having both normal body mass index and those who were overweight/obese. Anthropometric indices were assessed by employing standard protocols of corresponding body measurements while metabolic variables were determined by conducting relevant biochemical assays. The data related to lifestyle factors was collected by developing a standard questionnaire. Genotyping of rs7069102 was done by performing a TaqMan allelic discrimination assay. The data were analyzed by means of Statistical Package for the Social Sciences software by performing regression analyses adjusted for relevant confounders and corrected for multiple comparisons.<br /><strong>Results:</strong> The data analyses revealed that the interaction of SIRT1 rs7069102 with low physical activity (LPA) and also with irregular sleepwake cycle (SWC) influenced many anthropometric and metabolic indices such as weight, waist circumference, hip circumference, percent body fat, skinfold thicknesses (triceps, supra-iliac, sub-scapular, abdominal, biceps, and thigh), homeostatic model assessment for insulin resistance, fasting insulin, visceral adiposity index, lipid accumulation product and triglycerides (p < 0.05).<br /><strong>Conclusion:</strong> In conclusion, the interplay of the SIRT1 rs7069102 with LPA and also with irregular SWC may have a considerable impact on adiposity-associated anthropometric and metabolic outcomes in the local population of Pakistan.</p>