Abstract
The interaction between the diterpene, forskolin and prostaglandin E 1l on cAMP levels in isolated adipocytes of the rat was examined. Various doses of Prostaglandin E 1 in the presence of 1-methyl-3-isobutyl xanthine (2 mM) blocked the increase in the intracellular level of CAMP elicited by forskolin (0.1 PM or 5 PM). Interestingly, in the absence of 1-methyl-3-isobutylxanthine (or an adenosine blocker), or in the presence of 1-methyl-3-isobutylxanthine (2 mM) and forskolin (30 PM), prostaglandin E 1 (1 nM-100 μM) exhibited biphasic dose-response kinetics. The stimulatory effect of PGE 1 (> 1 μM) in presence of forskolin (30 μM may be a consequence of prostaglandin receptor modification following endogeneous release of adenosine and prostaglandins by adipocytes stimulated to high levels of cAMP accumulation due to 30 PM forskolin.
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